The effect of Terebinth oil on Ezrin and Moesin expression levels in rats with ovarian ischemia-reperfusion Injury

The objective of this study is to examine the expression levels of ezrin and moesin proteins from a molecular and immunohistochemical standpoint following the administration of terebinth oil in rats with ovarian ischemia- reperfusion injury. A total of 32 female Sprague-Dawley rats were utilized in the study. The rats were randomly assigned to one of four groups, with eight rats in each group: control, ischemia, I/R, and I/R+-terebinth oil. Following the induction of torsion, the treatment group received 2 ml.kg-1 of terebinth oil orally via gavage once daily for 28 d. At the conclusion of the experiment, ovarian tissues were obtained for immunohistochemical and molecular analysis. The immunohistochemical evaluation demonstrated a positive ezrin expression in epithelial cells within the I/R+-terebinth oil group, in comparison to the I/R group. Conversely, a negative reaction was observed in the vicinity of blood vessels. The expression of moesin was observed to be positive in granulosa cells and stromal areas. Additionally, a notable decline in the expression levels of ezrin and moesin proteins was observed in the treatment group in comparison to the damage group. Moreover, the administration of terebinth oil was observed to result in protein expression levels that were more closely aligned with those observed in the control group. The present study has demonstrated the impact of terebinth oil administration on the expression levels of ezrin and moesin proteins in a model of ovarian ischemia-reperfusion injury.