Long-lasting sex-specific alteration in left ventricular cardiac transcriptome following gamma and simGCRsim radiation
Space irradiation (IR) is an important health risk for deep-space missions. We reported heart failure with preserved ejection fraction like cardiac phenotype 660-days following exposure to a single-dose of a simplified galactic cosmic ray simulation (simGCRsim) only in males with functional and structural impairment in left ventricular (LV) function. This sex-based dichotomy prompted us to investigate sex-specific changes in the LV transcriptome in three-month-old male and female mice exposed to <sup>137</sup>Cs-γ- or simGCRsim-IR. Non-IR male and female (10 each) mice served as controls. LVs were collected at 440/660- and 440/550-days post-IR, male and female, respectively. RNA sequencing, differential gene expression, and functional annotation were performed on tissues from 5 mice/group. Sex and post-IR time points had the greatest influence on gene expression, surpassing the IR-type effects. SimGCRsim-IR showed more persistent transcriptome changes than γ-IR. We suggest that the single IR effects can persist up to 550-660 days, with overwhelmingly sex-biased responses at individual gene expression level.
Authors
- Suren Davitavyan (ORCID: https://orcid.org/0000-0002-3414-5553)
- Lahouaria Hadri (ORCID: https://orcid.org/0000-0001-8108-7993)
- Siras Hakobyan (ORCID: https://orcid.org/0000-0002-6875-2482)
- Susmita Sahoo (ORCID: https://orcid.org/0000-0002-7279-1564)
- Malik Bisserier (ORCID: https://orcid.org/0000-0001-8826-4649)
- Ani Stepanyan (ORCID: https://orcid.org/0000-0001-8564-8532)
- Tamara Sirunyan
- Agnieszka Brojakowska (ORCID: https://orcid.org/0000-0002-7319-9685)
- David A. Goukassian (ORCID: https://orcid.org/0000-0001-5270-5270)
- Arsen Arakelyan (ORCID: https://orcid.org/0000-0002-6851-1056)
- Mary K. Khlgatian
- Venkata Naga Srikanth Garikipati (ORCID: https://orcid.org/0000-0001-5803-5335)
- Gisane Khachatryan
- Roksana Zakharyan (ORCID: https://orcid.org/0000-0003-3411-7584)
- Shihong Zhang (ORCID: https://orcid.org/0000-0002-9661-1089)
Institutions
- New York Medical College (US)
- Russian-Armenian University (AM)
- Yale University (US)
- Institute of Molecular Biology (AM)
- Temple University (US)
- Icahn School of Medicine at Mount Sinai (US)
- National Academy of Sciences of Armenia (AM)
Publication Details
- Journal
- Scientific Reports
- Published
- 2025-02-18
- DOI
- https://doi.org/10.1038/s41598-025-89815-2
- Citations
- 2
- Primary Topic
- Genetics, Aging, and Longevity in Model Organisms
- Type
- article
- Field-Weighted Citation Impact
- 1.91
Funders
- American Heart Association
- National Institutes of Health