A Sensitive Ultrafiltration Plus RP-HPLC-FLD Method to Quantify Total and Free Perampanel Concentrations in the Plasma of Patients With Epilepsy: A Fully Validated Assay With Clinical Application
The method was rigorously validated for selectivity, accuracy, precision, and stability in accordance with established FDA and EMA guidelines. The developed method enables the rapid and accurate quantification of both total and free PER concentrations in the plasma of patients with epilepsy, offering technical support for its subsequent clinical application.
Authors
- Yilei Yang
- Jinjuan Liu
- Haiyan Shi (ORCID: https://orcid.org/0000-0002-7085-5413)
- Qiao-Yan Yi (ORCID: https://orcid.org/0000-0002-3262-0779)
- Jingya Xu
- Xueyan Cui
- Peng Wang (ORCID: https://orcid.org/0000-0001-8118-4929)
- Zhonghua Dong
Institutions
- Shandong University of Traditional Chinese Medicine (CN)
- Shandong Provincial QianFoShan Hospital (CN)
- Shandong First Medical University (CN)
Publication Details
- Journal
- Therapeutic Drug Monitoring
- Published
- 2025-02-18
- DOI
- https://doi.org/10.1097/ftd.0000000000001311
- Primary Topic
- Pharmacological Effects and Toxicity Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Natural Science Foundation of Shandong Province