Exploring the Roles of the Plant AT-Rich Sequence and Zinc-Binding (PLATZ) Gene Family in Tomato (Solanum lycopersicum L.) Under Abiotic Stresses

PLATZ transcription factors represent a novel class of zinc finger proteins unique to plants and play critical roles in plant growth and stress responses. This study performs a bioinformatic analysis on the PLATZ transcription factor family in tomato. In the tomato genome, 20 PLATZ transcription factors were identified, distributed across nine chromosomes, including two tandem duplication clusters and two segmental duplication events. Phylogenetic analysis classified tomato PLATZ family members into five subgroups, with consistent gene structures and motif distributions within the same subfamily. The stress-responsive and hormone signaling elements were widely distributed in the promoters of <i>SlPLATZs</i>. The qRT-PCR results showed that most tested <i>SlPLATZs</i> were highly expressed in flowers and significantly expressed under different abiotic stresses (PEG, low temperature, and salt treatments) and hormone treatments (ABA and SA). In addition, we determined that SlPLATZ13/17/18/19 showed transcriptional inhibitory activities via yeast and dual-luciferase reporter assays. The interactions between SlPLATZ17, SlDREB2, and SlDREB31 were preliminarily confirmed via yeast two-hybrid assays. Overall, this study provides a valuable theoretical foundation for functional function research on PLATZ transcription factors, particularly in response to abiotic stresses.