In vitro study of antioxidant and anti-inflammatory activities of Matricaria pubescens extracts

Our study is based on the evaluation of the biological activities of the aerial part of Matricaria pubescens, a medicinal plant of the traditional pharmacopoeia of Oued souf region. Two methods of extracting Soxhlet and maceration ethanolic. Phytochemical tests have revealed the presence of active ingredients, especially polyphenols and flavonoids. The Folin Ciocalteu method and AlCl3 aluminum trichloride, respectively determine total polyphenols and flavonoids. The results obtained indicate that the Soxhlet extract has the highest content of 59.25±0.27 µg gallic acid equivalent / mg of extract and 12.106 ±1.07 µg equivalent quercetin / mg of extract. While the low content present by the maceration 41.365±1.01 µg equivalent of gallic acid / mg of extract and 5.380 ± 0.085 µg equivalent quercetin / mg of extract. The evaluation of the anti-free radical activities by the free radical scavenging method (DPPH) has shown strong anti-free radical activities by Soxhlet, which is lower than that of the BHT with IC50 of 99.861±2.364 and 16.415±2.461 µg/mL respectively, compared to the maceration with IC50 of 106.340±2.407 µg/mL. The anti-inflammatory activity of the extracts was explored in vitro by the protein denaturation inhibition the test extracts showed inhibitory activity similar to diclofenac sodium with a percentage inhibition 67.038±1.36; 58.038±2.551 and 72.435±1.022 µg/mL, respectively.