Characterization of WA-CMS Rice Genotypes for Fertility Restorer and Submergence Tolerance Genes

Characterization of restorer lines form an imperative part of successful hybrid breeding programme in rice. Conventional methods of restorer lines characterization based on pollen and spikelet fertility is quite unreliable due to environmental factors. However, DNA based markers for the presence or absence of fertility restoration genes serve as an efficient, reliable, and rapid technique. In present investigation, 55 rice genotypes were characterized for fertility restorer (Rf) and submergence tolerance genes using QTL/gene based molecular markers. Marker analysis revealed that 46 genotypes have Rf4 gene, 51 genotypes have Rf3 gene, and 42 genotypes have both Rf4 and Rf3 genes while only 3 genotypes have Sub1 gene. Hence, these genotypes can be used as an effective restorer line in future rice hybrid breeding programmes depending upon the objective. Additionally, CR 2994-5-3-2-1-1, PAU 3207, and Swarna Sub1 can be utilized as restorers of male fertility breeding programmes orientated to produce submergence tolerant rice hybrids.