Efficacy of 810 nm Diode Low-Level Laser Treatment on Healing Periodontal Intrabony Defects: A Blinded Animal Investigation

Background: Low-power laser treatment demonstrated a biostimulatory positive impact during the initial phases of osseous regeneration. Purpose: The study's primary objective is to assess the histological impact of low-power laser therapy (810 nm) on healing intentionally generated intrabony periodontal defects. Methods: The investigation was evaluated by carrying out 16 identified defects involving Eight adult male rabbits (totalling 8 individuals) utilised in this study., categorised into 2 groups using the split-mouth technique. The control group defects were not subjected to any treatment modality, whereas the test group ones were subjected to laser irradiation. Four animals (8 defects) were euthanised after 15 days, and the other four (8 defects) at 30 days post-surgery, followed by histological analyses. Defects were bilaterally produced in each animal, manifesting as bony cavities of 10 mm in occlusoapical length and 4 mm in buccolingual length between the first and second molars, utilising a tapered drill (of fissure type) attached to a high-rpm motor. Following the randomised group allocation, the right-side defects were designated the control group, while the left ones were subjected to laser irradiation. Results: Irradiated defects exhibited diminished inflammatory response, advanced periodontal fibre regeneration and conjunction, and developed bone growth after 15 and 30 days compared to the untreated control group. Conclusions: Low-power laser therapy diminishes the inflammatory response, enhances periodontal fibre regeneration and alignment, and promotes bone maturation and healing. Keywords: Low-Power Laser Therapy, Biostimulation, Periodontal Intrabony Defects, Periodontal Regeneration, Rabbits, Histological Study