Content of BB platelet growth factor in platelet lysates obtained from donor blood

Cell therapy is among the main methodological approaches in regenerative medicine. Its effectiveness is determined by the use of cell cultures containing the largest available number of viable cells. An important role in this process is played by growth factors that ensure cell proliferation and differentiation. Both recombinant proteins and native proteins from various biological fluids may be used as growth factors. Blood platelets, being anucleate cells, are a promising source of growth factors. Along with their main blood clotting function, the platelets contain a number of unique biological molecules in alpha granules, i.e., mediators involved in immune response, inflammation and regeneration processes. The major families of growth factors present in platelets are: platelet-derived growth factors (PDGF), transforming growth factors (TGF-β), vascular endothelial growth factors (VEGF), epithelial growth factors (EGF), fibroblast growth factors (FGF), insulin-like growth factors (IGFs). The PDGF family includes several subtypes: PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC, PDGF-DD. PDGF-BB has high angiogenic, chemotactic and mitogenic activity. It takes part in the proliferation and migration of interstitial cells in various organs, proliferation of smooth muscle cells in respiratory tract, development of cerebral vessels and renal glomeruli. The aim of our study was to examine the content of PDGF-BB factor in platelet lysates obtained from healthy donors. Platelet lysate is a complex cocktail containing many growth factors, cytokines, and chemokines. The concentration of specific factors in final product obtained from platelet concentrate is different for each donor, and, apparently, depends on age, gender, blood type and other individual differences. When choosing a growth additive for a cell culture, the cell biologist should be focused on high content of the trophic factor that most closely matches the goals of the given experiment. The use of recombinant additives in the culture medium is justified by precise dosage of these components. However, the use of natural sources may be more preferable, due to presence of different growth factors required for in vitro expansion of highly cellular viable cultures. According to our results, the optimal additive for obtaining viable fibroblasts, endothelial cells, mesenchymal multipotent stem cells is a growth-inducing product obtained from platelet lysate of female donors of Bα (III) group, and male donors of Aβ (II) blood groups which contain the highest concentration of PDGF-BB.