Monitoring the Formation of Microcrystal Suspensions of Proteins Used in Serial Microcrystallography by Small-Angle X-ray Scattering

Serial crystallography, which has been extensively developed in the past years, makes it possible to study dynamic processes occurring in protein molecules. The method is implemented at fourth-generation synchrotron sources or free electron lasers and it requires suspensions of microcrystals. It was demonstrated that small-angle X-ray scattering can be used to monitor the formation of such suspensions in crystallization conditions as exemplified by lysozyme.