MicroRNA-27a transfected dental pulp stem cells undergo odonto/osteogenic differentiation via targeting DKK3 and SOSTDC1 in Wnt/BMP signaling in vitro and enhance bone formation in vivo
Transfection of miRNA-27a in hDPSCs enhanced their odonto/osteoblastic differentiation by targeting DKK3 and SOSTDC1, thereby promoting the Wnt and BMP signaling. Transplantation of miRNA-27a-overexpressing hDPSCs promoted new bone formation in vivo. These findings deepen our understanding of the effects of miRNA on Wnt and BMP pathways and suggest a potential clinical application for miRNA-27a in promoting hard tissue regeneration, offering a promising therapeutic target for dental and craniofacial tissue reconstruction.
Authors
- Nobuyuki Kawashima (ORCID: https://orcid.org/0000-0002-1636-0487)
- Keisuke Nara (ORCID: https://orcid.org/0000-0002-1023-1529)
- Shihan Wang (ORCID: https://orcid.org/0009-0000-9926-9533)
- Peifeng Han (ORCID: https://orcid.org/0000-0002-1442-9102)
- Ziniu Yu (ORCID: https://orcid.org/0009-0005-0533-5664)
- Kento Tazawa (ORCID: https://orcid.org/0000-0001-9725-6034)
- Sonoko Noda (ORCID: https://orcid.org/0000-0001-9981-8344)
- Takashi Okiji (ORCID: https://orcid.org/0000-0002-9791-4508)
- Thoai Quoc Kieu (ORCID: https://orcid.org/0000-0001-5541-1614)
- Ren Chunmei
Institutions
- Tokyo Medical and Dental University (JP)
- University of Medicine and Pharmacy at Ho Chi Minh City (VN)
Publication Details
- Journal
- Journal of Translational Medicine
- Published
- 2025-02-16
- DOI
- https://doi.org/10.1186/s12967-025-06208-9
- Citations
- 5
- Primary Topic
- MicroRNA in disease regulation
- Type
- article
- Field-Weighted Citation Impact
- 4.68
Funders
- Tokyo Medical and Dental University
- Japan Society for the Promotion of Science
- Japan Science and Technology Agency