Elastic vesicles for enhancing the topical repurposed effect of Propranolol against Candida Albicans

The goal of this investigation was to load transferosomes with the repurposed antifungal drug propranolol to treat candida albicans topically. The production of transferosomes for enhancing propranolol topical administration was the focus of this study. By a 21.31 full-factorial experiment with different surfactants and phospholipid amounts, transferosomes formulas were fabricated. Transferosomes were assembled by ethanol injection approach. Transferosmes were evaluated for their entrapment efficiency (EE%), particle size (PS), polydispersity index (PDI), and zeta potential (ZP). Employing Design Expert® software, the optimum-formula (F3) was opted, revealing ZP of -28.15±1.11 mV, PDI of 0.476±0.001, PS of 207.05±0.45nm, and EE% of 71.00±0.94%. Further, the optimum formulation illustrated spherical transferosomes with no aggregation under transmission electron microscope inspection. In-vitro release investigation verified that the optimum formula was released in a sustained manner than propranolol solution. In addition, during storage the optimum formula was stable. The histological inspection confirmed the tolerability of the optimum transferosomes.