Antibiogram and Molecular Detection of Klebsiella pneumoniae Biofilm-Forming Genes Isolated From Meat Products in Qalyubia Governorate, Egypt

Incidence of foodborne pathogens in meat and meat products poses significant health risks to humans and results in financial losses for producers due to market recalls. This study aimed to isolate and identify of pathogenic Klebsiella pneumoniae from meat and its products by using conventional culture and biochemical characters. In addition, molecular technique was applied for detection of the isolated Klebsiella pneumoniae using gapA gene. Overall, total of one hundred (n=100) random samples of beef products including 10 from each {minced meat, kofta, sausage, luncheon, burger, bastrma, frankfurter, liver, chicken and shawarma} were collected from Benha markets in Qalubia Governorate, Egypt, for bacteriological examination. The antimicrobial resistances were investigated against 7 different classes of antimicrobial groups. The results showed that incidence of isolated from meat products were 10 isolates Klebsiella. The antimicrobial sensitivity test revealed that high sensitivity to imipenem and gentamicin and high resistance to cefotaxime, levofloxacin, amoxicillin & clavulanic acid, erythromycin and trimethoprime & sulfonamide. Further, molecular identification of gapA gene revealed that 5 Klebsiella pneumoniae at about 391 bp. Also, screening of 3 virulence genes (mrkD, uge, luxS) of 5 K. pneumoniae (n=5) which responsible for biofilm production showed the positive result at (226pb, 535pb, 477pb) for the examined strains. Finally; this study concluded that meat products have significant health hazards due to bad sanitary quality, increase the load of pathogenic Klebsiella and the presence of multidrug resistant (MDR).