Water sample stability

Numerous guidelines have been issued on the conditions under which water samples should be transported and stored before microbiological analysis. These recommendations are often very restrictive regarding the maximum acceptable storage time (MAST) of samples before analysis and they generate operational constraints that have significant economic and environmental consequences (e.g. samples rejected, unjustified prolongation of quarantine of a water supply point or medical device, recontrol,…). Unfortunately, the relevance of these MAST has never been demonstrated. This study investigates bacterial flora evolution in water samples according to their storage time before analysis. The first part, conducted in laboratory conditions follows the concentration of microorganisms in artificially contaminated water samples. The second part involves a retrospective analysis of real water sample results to verify whether the results varied statistically according to the sample storage time before analysis. The results of the laboratory study confirm that the microorganism concentration in the artificially contaminated samples vary by less than 25 % during the storage time. The second part of the study demonstrates that the ratio of samples belonging to the different result groups (e.g. < 1 CFU/ X mL, between 1 and 99 CFU/X mL and ≥ 100 CFU/100 mL) remains unchanged according to storage time (variation < 1 %). These findings confirm that it is possible to increase the MAST defined in standards, up to 26 h or 28 h according to the microorganism considered (instead of 12 h or 18 h) without compromising the validity of microbiological analysis. These changes should limit the operational, environmental, and economic constraints associated with the actual MAST by reducing the number of samples rejected and consecutively the number of recontrols. • The relevance of actual recommendations regarding water samples transportation and storage before analysis has never been demonstrated. • Data demonstrate that there is no statistical difference in the concentration of microorganisms found in samples maintained at 5°C for 18 h or 26 h . • The in-use test, demonstrated that the sample storage time had little or no influence on the results of water microbiological analysis. • It is possible to increase the maximum sample storage times recommended without compromising the validity of microbiological analysis.