Gibberellin biosynthesis gene ScGA20 oxidase enhances sugarcane growth by modulating genes associated with phytohormone and growth processes

Sugarcane is a globally significant crop for sugar and energy production, yet its breeding potential is limited by its complex genetic background and restricted genetic diversity. Molecular breeding presents a promising approach to boost sugarcane productivity. Gibberellins (GA) plays a critical role in plant growth and development, with GA20-oxidase being the most crucial enzyme in GA biosynthesis. In this study, we isolated the sugarcane gene encoding GA20-oxidase (ScGA20ox, OR283803) enzyme, a 43.88 kDa hydrophilic protein, and introduced it into sugarcane variety GT42 via biolistics transformation. Transgenic sugarcane expressing Ubi-driven ScGA20ox exhibited elevated GA levels and accelerated growth. Transcriptome analysis revealed that genes associated with plant hormone metabolism and growth-related pathways, including photosynthesis, plant hormone signal transduction, and starch and sucrose metabolism were involved in the GA20ox-mediated growth in sugarcane. This work underscores the potential of GA20ox for enhancing sugarcane yields through transgenic approaches, while advancing our understanding of GA's regulatory role in the growth of this crucial sugar crop.