Endemic Dactylorhiza osmanica subsp. osmanica: Ex vitro symbiotic germination approach

In this study, the potential effects of Ceratobasidium sp. fungus on the ex vitro symbiotic germination of Dactylorhiza osmanica (Klinge) P. F. Hunt & Summerh subsp. osmanica seeds were evaluated. Results, Ceratobasidium sp. caused that about 51.95% germination rate was obtained in seeds inoculated. Protocorm and primordium formation rates were determined to be 25.27% and 26.67%, respectively. These findings indicate that the fungus promotes the germination of seeds. Ceratobasidium sp. on ex vitro symbiotic seed germination of D. osmanica subsp. osmanica shows the potential effect of the fungus Longer term and more detailed studies should be conducted to reach adult plants and adapt to natural conditions. These findings are very promising for protecting of endemic and rare orchid species.