Effective Control and Eradication of Mycoptes musculinus in Research Mice Colonies

Aims: To develop and evaluate an effective eradication program for Mycoptes musculinus in a research facility by implementing a combination of animal isolation, environmental control, and chemical treatments. Study Design: Observational case study documenting the identification, control, and eradication phases of a mite infestation in a laboratory setting. Place and Duration of Study: Institute Animal facility, IISER Bhopal, conducted over three months (e.g., August 2024 to October 2024). Methodology: Clinical signs of infestation, such as excessive grooming, hair loss, and scaling, prompted diagnostic tests including cellophane tape tests, skin scrapings, and PCR for confirmation. Microscopic examination identified mite sexual dimorphism, aiding in understanding population dynamics. Control measures included isolating and euthanizing affected mice, sealing wild rodent access points with plaster of Paris, and disinfecting facility areas with ivermectin (1:50 dilution) and Butox solution (5 ml/L). Weekly follow-ups and environmental inspections ensured the effectiveness of the intervention. Results: The intervention and butox therapy successfully eradicated Mycoptes musculinus from the facility. Post-treatment follow-ups showed no evidence of reinfestation over a three-month period. Environmental sealing, combined with strategic disinfection protocols, proved effective in breaking the infestation cycle. Conclusion: A swift and integrated response, including animal containment, environmental controls, and chemical disinfection, can eradicate Mycoptes musculinus in laboratory facilities. This case underscores the importance of early detection, comprehensive mitigation strategies, and continuous monitoring in maintaining biosecurity in research animal facilities. Future research should focus on alternative and sustainable approaches to prevent mite outbreaks.