Humic nanoparticles as a tool for eliminating the toxicity of zinc L-valinate

Earlier, we showed (Morozova M.A. et al., 2022) that solutions of the humic acids (HAs) and fulvic acids (FAs) complex used as a solvent are applicable for increasing the solubility and colloidal stability of antiviral drugs. In this study, we investigated the ability of humates to form stable colloidal systems with chelated zinc complexes with amino acids. The study of the dispersion properties of the samples was carried out using the dynamic light scattering (DLS) technique. There were obtained HAs and FAs dilutions that form a colloidal solution with zinc chelate complexes with specified characteristics of particle size and zeta potential. The solutions contained mainly 20 nm nanoparticles and their zeta potential was -24 mV. The toxicity was assessed using the Spirotox method. A comparative toxicological analysis of zinc valinate samples dissolved in HAs and water showed that the use of HAs as an adjuvant allows for a radical decrease in the toxicity of zinc chelate. Keywords: extract of humic substances; fulvic acid; zinc chelate complexes; zincvalinate; toxicity; Spirotox-method