Synergistic Anti-Migration Effects of Garcinia cowa and Doxorubicin in T47D Breast Cancer Cells: A Scratch Assay Analysis

Objective: Breast cancer metastasis is a major cause of mortality, highlighting the need for effective anti-migratory therapies. This study investigated the synergistic anti-migratory effects of Garcinia cowa bark ethanol extract and doxorubicin on T47D breast cancer cells, aiming to explore its potential as a combination therapy to inhibit cancer cell migration Methods: T47D cells were treated with Garcinia cowa bark ethanol extract (GCBEE) (130 µg/mL), doxorubicin (Dox) (0.026 µg/mL), and their combination. Cell migration was evaluated using the scratch assay, with scratch closure monitored at 0, 24, and 48 hours. Results: The combination of GCBEE (130 µg/mL) and Dox (0.026 µg/mL) significantly inhibited T47D cell migration at both 24 and 48 hours, compared to the individual treatments of DOx (0.026 µg/mL) alone and GCBEE (130 µg/mL) alone. Conclusions: The combination of Garcinia cowa ehtanol extract and doxorubicin demonstrates synergistic anti-migratory effects on T47D breast cancer cells, suggesting its potential as an adjuvant therapy to enhance the efficacy of doxorubicin in preventing metastasis. Keywords: Garcinia cowa, doxorubicin, T47D cells, anti-migration, scratch assay, combination therapy.