Effect of Salvia przewalskii root extract on ethanol voluntary intake and mRNA expression of GABAA receptor subunits in the brain of alcohol-preferring rats
Introduction: Literature data and our research have shown that extracts from the underground plant parts of Salvia przewalskii (SP) reduce alcohol intake in animal models. Objective: The study aimed to assess the effect of SP on mRNA expression of GABAA receptor (GABAAR) subunits (alpha 1–4, delta, gamma 2L, gamma 2S) genes in the brain of ethanol-drinking rats. Methods: The investigations were performed on Warsaw high-alcohol-preferring (WHP) and low-alcohol-preferring (WLP) rats treated with the hydroalcoholic (1:1) extract from SP (150 mg/kg, p.o.) for 28 consecutive days. The mRNA expression was measured by RT-PCR analysis in the cortex, striatum, and hippocampus. Results: SP lowered the alcohol intake in WHP animals. The extract down-regulated alpha and gamma subunit expression in WHP rats mainly in the hippocampus, but simultaneously, the delta subunit upregulation was observed. Conclusions: SP’s effect on drinking behavior can be coupled with the modulation of GABAAR subtype expression in the brain of rats.
Authors
- Artur Adamczak (ORCID: https://orcid.org/0000-0002-1738-953X)
- Edyta Wyszogrodzka (ORCID: https://orcid.org/0000-0001-9193-368X)
- Radosław Kujawski (ORCID: https://orcid.org/0000-0002-2389-0689)
- Waldemar Buchwald (ORCID: https://orcid.org/0000-0002-4180-510X)
- Kamila Czora-Poczwardowska (ORCID: https://orcid.org/0000-0003-2873-4746)
- Monika Karasiewicz (ORCID: https://orcid.org/0000-0002-8373-037X)
- Joanna Bartkowiak-Wieczorek (ORCID: https://orcid.org/0000-0002-8347-0512)
- Michał Szulc (ORCID: https://orcid.org/0000-0003-3295-2789)
- Anna Bogacz (ORCID: https://orcid.org/0000-0001-5455-8315)
- Marcin Ożarowski (ORCID: https://orcid.org/0000-0003-2305-3116)
- Wanda Dyr (ORCID: https://orcid.org/0000-0002-5741-308X)
- Przemysław Ł. Mikołajczak (ORCID: https://orcid.org/0000-0002-1252-6523)
- Alina Szydło
- Agnieszka Gryszczyńska (ORCID: https://orcid.org/0000-0002-8164-9914)
- Ewa Kamińska (ORCID: https://orcid.org/0000-0002-7708-0139)
- Rami Taqtaq
Institutions
- Poznan University of Medical Sciences (PL)
- Heliodor Swiecicki Clinical Hospital (PL)
- Institute of Natural Fibres and Medicinal Plants (PL)
- Institute of Psychiatry and Neurology (PL)
Publication Details
- Journal
- Herba Polonica
- Published
- 2024-12-31
- DOI
- https://doi.org/10.5604/01.3001.0054.8770
- Primary Topic
- Traditional Chinese Medicine Analysis
- Type
- article
- Field-Weighted Citation Impact
- 0.00