Phytochemical Fingerprinting of a pharmacopoeial Unani formulation Habb e Asgand by TLC and HPLC for Standardization

Habb-e- Asgandh a pharmacopoeial Unani preparation used for treating joints pain was formulated and then extracted in absolute ethanol and with the 50% alcohol. After drying these extracts, HPLC & TLC fingerprints were developed. TLC studies of Ethanolic and Hydroalcoholic extract of the formulation were carried out using two mobile phases (1) Toluene, Chloroform, Acetone and Acetic acid (70:15:15+2 drops) and (2) Benzene and Ethyl acetate (90:10). Thereafter, the TLC plates were observed in daylight, UV long and short wave length, and also treated with Iodine vapours and Anisaldehyde spray. Different numbers of spots were observed and Rf values alongwith colour were documented. HPLC of Ethanolic and Hydroalcoholic extract of Habb-e- Asgnadh was also carried out at 254 nm wavelength and the chromatogram was obtained. The mobile phase and flow rate was HPLC grade methanol and 1ml/min respectively. The HPLC profile of the Ethanolic extract of the test formulation gives 21 peaks and of the Hydroalcoholic extract of the formulation depicted total 6 peaks The TLC and HPLC profile of the test formulation can be used for standardization and quality control for future batches of Habb-e- Asgnadh.