Screening of Native Isolates of Trichoderma spp. and Pseudomonas fluorescens against Rhizoctonia solani under In vitro Condition

Production and inhibitory effect of volatile compounds of Trichoderma isolates and Pseudomonas fluorescens were tested by dual culture technique against the Rhizoctonia solani. Trichoderma isolates were isolated from various places of Rajnandgaon, Raipur and Durg district of Chhattisgarh. The study about screening of native isolates of Trichoderma harzianum aginst R. solani in vitro revealed that all native isolates of T. harzianum were significantly inhibited the mycelial growth of R. solani. The maximum reduction in mycelial growth of R. solani from volatile compounds was recorded in Th-4 followed by Th-1, Th-2, Th-5 and Th-3. The study of native P fluorescens on mycelial growth and sclerotial formation of R solani revealed that all P. fluorescens isolates significantly inhibited the mycelial growth of R. solani. The mycelial growth of R. solani was highly inhibited by Pf-05 isolate (with 7.94mm mycelial growth and 91.18% decrease over control). The study about screening of native bioagents, Trichoderma and Pseudomonas revealed that the bioagent isolates significantly reduced the mycelial growth of R. solani under In vitro.