Precision Unveiled: Robust RP-HPLC Method for Accurate Estimation of Prasugrel, a Platelet Aggregation Inhibitor, in Final Dosage Form

Background: Administered orally, Prasugrel is a third generation thienopyridine that irreversibly inhibits platelet aggregation by binding with P2Y12 receptor. It is available in coated tablets of 5 and 10 mg commercially, but there are very few analytical methods available for estimation of Prasugrel in final dosage form. Objective: The current study introduces a simple and accurate high-performance liquid chromatographic technique to determine the amount of Prasugrel in the final dosage form Method: The mobile phase consists of Methanol – Buffer (700 + 300, v/v). A column containing octadecylsilane chemically bonded to porous silica particles (Purospher star ® 100 ODS 250 x 4.6 mm, 5 µm) was used as stationary phase. Detection was performed using a variable wavelength ultraviolet-visible detector set at 220 nm for Prasugrel. Solutions were injected into the chromatograph under isocratic condition at a constant flow rate of 1.0 mL/min. The stability indicating capability of the method was confirmed through a force degradation study.