Metabolite Profiling Based UHPLC‐HRMS of Indonesian <i>Peperomia pellucida</i> and Its Phytochemical, Antioxidant, and Enzyme Inhibitory Properties

Abstract One of promising Indonesian botanicals, Peperomia pellucida , lacks a standardized extraction technique due to its wide range of substances. In this study, the phytochemicals, antioxidants, and enzyme inhibitory activities of P. pellucida were examined after being extracted using UAE with 70% ethanol and partitioned using the solid–liquid partition technique with four solvents of variable polarity. Metabolites composition in P. pellucida was identified using UHPLC‐HRMS, further assessed with chemometric analysis for classifying the data. The evaluation on phenolics, flavonoids, terpenoids, and tannins contents presents the strong correlation with its antioxidant activity (DPPH, FRAP, ABTS) as well as the enzyme inhibition (lipase, tyrosinase, and α ‐glucosidase). The metabolomic analysis revealed that tetrahydrofolic acid, diosmin, 7‐hydroxy‐3‐(4‐methoxyphenyl)‐4‐propylcoumarin, (‐)‐caryophyllene oxide, hesperetine, and 6β ‐naltrexol were the major constituents contained in P. pellucida extract, while amino acids, flavonoids, polyphenols, and terpenoids were the most abundant active class presence in the extract. The PCA and PLS‐DA successfully classified P. pellucida extract and fractions into five groups.