Reduced RG-II pectin dimerization disrupts differential growth by attenuating hormonal regulation
Defects in cell wall integrity (CWI) profoundly affect plant growth, although, underlying mechanisms are not well understood. We show that in <i>Arabidopsis mur1</i> mutant, CWI defects from compromising dimerization of RG-II pectin, a key component of cell wall, attenuate the expression of auxin response factors <i>ARF7-ARF19</i>. As a result, polar auxin transport components are misexpressed, disrupting auxin response asymmetry, leading to defective apical hook development. Accordingly, <i>mur1</i> hook defects are suppressed by enhancing <i>ARF7</i> expression. In addition, expression of brassinosteroid biosynthesis genes is down-regulated in <i>mur1</i> mutant, and supplementing brassinosteroid or enhancing brassinosteroid signaling suppresses <i>mur1</i> hook defects. Intriguingly, brassinosteroid enhances RG-II dimerization, showing hormonal feedback to the cell wall. Our results thus reveal a previously unrecognized link between cell wall defects from reduced RG-II dimerization and growth regulation mediated via modulation of auxin-brassinosteroid pathways in early seedling development.
Authors
- Jinxing Lin (ORCID: https://orcid.org/0000-0001-9338-1356)
- Pawan Kumar Jewaria (ORCID: https://orcid.org/0000-0002-1961-3750)
- Bibek Aryal (ORCID: https://orcid.org/0000-0003-0840-6956)
- Stephen C. Fry (ORCID: https://orcid.org/0000-0002-1820-4867)
- Kristoffer Jonsson (ORCID: https://orcid.org/0000-0001-7445-7378)
- Rishikesh P. Bhalerao (ORCID: https://orcid.org/0000-0003-4384-7036)
- Eugenia Russinova (ORCID: https://orcid.org/0000-0002-0569-1977)
- Rifat Ara Begum
- Stéphane Verger (ORCID: https://orcid.org/0000-0003-3643-3978)
- Gloria Sáncho-Andrés (ORCID: https://orcid.org/0000-0001-6759-8543)
- Xiaojuan Li (ORCID: https://orcid.org/0000-0002-9955-2467)
- Abu Imran Baba (ORCID: https://orcid.org/0000-0002-2703-6308)
- Meng Yu (ORCID: https://orcid.org/0009-0005-4534-424X)
- Yaowei Wang (ORCID: https://orcid.org/0000-0002-1001-2342)
Institutions
- University of Dhaka (BD)
- Espace pour la vie (CA)
- Beijing Forestry University (CN)
- Ghent University (BE)
- ETH Zurich (CH)
- VIB-UGent Center for Plant Systems Biology (BE)
- Umeå Plant Science Centre (SE)
- National Institute of Plant Genome Research (IN)
- Hebei University (CN)
- University of Edinburgh (GB)
Publication Details
- Journal
- Science Advances
- Published
- 2025-02-12
- DOI
- https://doi.org/10.1126/sciadv.ads0760
- Citations
- 5
- Primary Topic
- Plant Molecular Biology Research
- Type
- article
- Field-Weighted Citation Impact
- 8.75
Funders
- Knut och Alice Wallenbergs Stiftelse
- Vlaamse regering
- Beijing Forestry University