Effect of Ulva fasciata powder as biostimulant on Sedum praealtum in vitro growth

Biostimulants are considered as biological, organic and synthetic components that promote the plant growth and also they have been used as an additive for improving in the plant tissue culture technology the successful initiation, regeneration and micropropagation of in vitro culture of higher plants The aim of the present work was to analyze the effect of Ulva fasciata seaweed powder on the growth and differentiation of Sedum praealtum in vitro culture. The measurements of the area and perimeter descriptors showed a particularly positive effect at 0.25% U. fasciata powder, followed by the experimental condition of culture with 0.5% U. fasciata powder with more area and perimeter than control explants, respectively with statistical significance. Regarding to the morphometric relationship between aspect ratio (AR) and roundness of the explants, all of the experimental conditions with and without the U. fasciata powder indicated that all of S. praealtum explants were closer to the ellipsoidal values than to the roundness. Also in this study, not only the nature of phytohormones present in the media tested (naphthalene acetic acid and kinetin) for S. praealtum explants culture were determinant; also the addition of seaweed powder may be promoted the growth of them and also an initial changes of morphogenesis with the evidence of an incipient callus.