The Combined Effect of Arbuscular Mycorrhizal Fungi and Arsenic Doses on Oxidative Stress of Leuceana leucocephala in Nursery

The aim of this research was to examine the effect of high levels of arsenic in soil, on L. leucocephala seedlings in nursery, an important small tree for forage and fodder purpose. Arbuscular mycorrhizal fungus (AMF) can shield host plants from the toxicity of arsenic (As). However, there is currently little known about how woody legumes react to As stress. Antioxidants activity to oxidative stress were evaluated in Leuceana leucocephala subjected to various doses of arsenic toxicity and also inoculation with arbuscular mycorrhizal fungi (AMF) in order to evaluate its effect on protection against oxidative stress and its tolerance after mycorrhizal inoculation. In order to evaluate this, MDA, H2O2, APX, CAT, GPX and GR. Exogenous application of arsenic results in negative effect on growth by increasing the production of Reactive oxygen species (ROS). While inoculation with Mycorrhizal fungi an enhancement in growth and other characters were noticed. This suggests that the use of these stress-relieving substances (AMF) may have reduced oxidative stress in As-stressed L. leucocephala seedlings. Accumulation of arsenic and phosphorus was increased by AMF inoculation in Root of seedlings.