Curative Effect of Total Moringa oleifera Leaf Extract on Reticulocyte Parameters in Anemic Rats

This study aims to evaluate the evolution of reticulocyte parameters in rats made anemic and treated with an aqueous extract of Moringa oleifera leaves. A total of 70 rats aged 8 to 16 weeks were divided into seven groups of ten rats each, composed equally of males and females. Anemia was induced by intraperitoneal injection of 40 mg/kg body weight of phenylhydrazine (PHZ), administered twice a day for two days. Different doses of the aqueous extract (200 mg/kg, 400 mg/kg, 800 mg/kg, and 1600 mg/kg body weight) were administered, compared to a positive control group and a group treated with Ranferon®. Treatments were administered from day 3 to day 14, with blood samples taken on days 1, 3, 7, and 14. The reticulocyte parameters evaluated include reticulocyte count, immature reticulocyte fraction (IRF), reticulocyte hemoglobin content (Ret-He), reticulocyte production index (RPI), or corrected reticulocyte count (CRC). The results indicate significant correction of anemia by the aqueous extract of Moringa oleifera leaves as early as the seventh day of the experiment. The mean hemoglobin level of rats treated with the Moringa extract was 14.21±0.07 g/dL, and the mean reticulocyte rate was 4.27±0.30 %, unlike the positive control, which had a hemoglobin level of 10.8±0.46 g/dL and a reticulocyte rate of 8.76±0.39 %.