Antibacterial Efficacy of Pure Aloe Vera, Methanol Extract and Gentamicin Against Pathogenic Bacteria

The Aloe vera plant has been utilized for thousands of years for traditional medicinal reasons, including the prevention of bacterial development. Objectives: To investigate the antibacterial effectiveness of pure Aloe vera and its methanol extract, comparing their efficacy to that of the commonly used antibiotic gentamicin. Methods: 50 broiler meat samples were collected from the butcher markets of Tandojam. Isolation and identification of the microorganisms, the minimum inhibitory concentration (MIC) was done on 96 well plates. For this purpose, similar concentrations of pure Aloe vera, its methanol extract and gentamicin were to evaluate their minimum inhibitory concentration against Staphylococcus aureus and Escherichia coli. The minimum inhibitory concentration was tested based on the transparency and turbidity of the medium. Results: Out of 50 meat samples identified 19 (38%) were found positive for Staphylococcus aureus and 24 (48%) were positive for Escherichia coli. The mean susceptibility value of Staphylococcus aureus was noticed at 10, and 20µg/µl for pure Aloe vera, 20, 10, 5µg/µl for methanol extract and 20, 10, 5, 2.5, 1.25µg/µl for gentamicin. While the mean concentration at which Escherichia coli growth inhabited was 20 µg/µl and 20, 10 µg/µl, and 20, 10, 5, 2.5, 1.25, 0.625, 0.312 µg/µl for pure Aloe vera, its methanol extract and gentamicin, respectively. Conclusions: It was concluded that all treatments i.e., pure Aloe vera, its methanol extract, and gentamicin inhibited the growth of isolated bacterial organisms, but the methanol extract stopped the growth of isolated organisms at lowered concentration in comparison to pure aloe vera.