Green Extraction of Antioxidant-Rich Flavonoids from Fagonia cretica Using Deep Eutectic Solvents

This study optimized the extraction of flavonoids from <i>Fagonia cretica</i> using deep eutectic solvents (DESs), focusing on key factors such as the type of DES used, molar ratio, water content, solid/liquid ratio, extraction temperature, and time. Among six DESs tested, the betaine-acetic acid combination exhibited the highest extraction efficiency, attributed to its low viscosity (4.98 mPa·s). Optimal extraction conditions were determined to be a 1:4 molar ratio of betaine to acetic acid, a 25% water content, a solid/liquid ratio of 1:60 g/mL, an extraction temperature of 50 °C, and an extraction time of 30 min. Under these conditions, the flavonoid yield was maximized while preserving bioactivity. Antioxidant assays revealed that flavonoids extracted with DESs exhibited superior scavenging activity against DPPH and hydroxyl radical compared to ethanol-extracted flavonoids, highlighting DESs' potential to enhance antioxidant properties. The recyclability of DESs was demonstrated using ultracapacitor porous activated carbon, achieving an 89.78% recovery efficiency. The reused DES maintained a high flavonoid extraction yield, retaining 92% efficiency after six cycles, emphasizing its sustainability and cost-effectiveness. This study establishes DES-based extraction as an environmentally friendly and efficient approach for isolating flavonoids with strong antioxidant properties, offering significant advantages in green chemistry and bioactive compound recovery.