Accuracy of capillary blood sampling for diagnosing syphilis infection

Capillary blood collection presents advantages such as reduced invasiveness over venous serum for syphilis diagnosing. This study aimed to compare diagnostic accuracy between capillary and venous blood samples for syphilis diagnosis. Individuals aged ≥ 18 years were included in a cross-sectional study. Syphilis screening was done using Rapid tests (RT) followed by collection of serum capillary and venous samples for VDRL and TPHA test. Sensitivity, specificity, and Kappa coefficient were calculated. Of 191 participants, 115 RT + and 76 RT-. Diagnostic properties did not significantly differ between capillary and venous samples. Capillary VDRL showed 99% sensitivity and 100% specificity, mirroring TPHA results. Furthermore, there was significant agreement between sample types for both serological tests (p < 0.001). Capillary sampling offers comparable diagnostic accuracy to venous collection, regardless of serum quality. Capillary sampling holds promise, particularly in developing countries and large-scale testing initiatives.

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Publication Details

Journal
Scientific Reports
Published
2025-02-12
DOI
https://doi.org/10.1038/s41598-025-88329-1
Citations
1
Primary Topic
Syphilis Diagnosis and Treatment
Type
article
Field-Weighted Citation Impact
1.16

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article

Accuracy of capillary blood sampling for diagnosing syphilis infection

Eliana Wendland, Vinícius Serafini Roglio, Aniúsca Vieira dos Santos, Luana Giongo Pedrotti et al.
1 citations
Scientific Reports
Syphilis Diagnosis and Treatment
1.16
article

Accuracy of capillary blood sampling for diagnosing syphilis infection

Eliana Wendland, Vinícius Serafini Roglio, Aniúsca Vieira dos Santos, Luana Giongo Pedrotti, Gérson Fernando Mendes Pereira, Suélen Porto Basgalupp, Emerson Silveira de Brito, Flávia Moreno Alves de Souza, Ana Carolina Monteiro da Rocha, Thayane Martins Dornelles, Cáren Nunes de Oliveira, Giovana Tavares dos Santos, Igor Araujo Vieira
article en
1 citations

Abstract

Capillary blood collection presents advantages such as reduced invasiveness over venous serum for syphilis diagnosing. This study aimed to compare diagnostic accuracy between capillary and venous blood samples for syphilis diagnosis. Individuals aged ≥ 18 years were included in a cross-sectional study. Syphilis screening was done using Rapid tests (RT) followed by collection of serum capillary and venous samples for VDRL and TPHA test. Sensitivity, specificity, and Kappa coefficient were calculated. Of 191 participants, 115 RT + and 76 RT-. Diagnostic properties did not significantly differ between capillary and venous samples. Capillary VDRL showed 99% sensitivity and 100% specificity, mirroring TPHA results. Furthermore, there was significant agreement between sample types for both serological tests (p < 0.001). Capillary sampling offers comparable diagnostic accuracy to venous collection, regardless of serum quality. Capillary sampling holds promise, particularly in developing countries and large-scale testing initiatives.

Scientific ReportsVol. 15(1)
Universidade Federal do Rio Grande do Sul (BR), Universidade Federal de Ciências da Saúde de Porto Alegre (BR), Hospital Moinhos de Vento (BR), Department of STD, AIDS and Viral Hepatitis (BR)
Ministério da Saúde
Zero hunger
Openalex Percentile: Top 26%
Syphilis Diagnosis and Treatment
1.16
DOIOpen Access PDFOpenAlex
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