Functionally active modulators targeting the LRRK2 WD40 repeat domain identified by FRASE-bot in CACHE Challenge #1
Critical Assessment of Computational Hit-Finding Experiments (CACHE) Challenges emerged as real-life stress tests for computational hit-finding strategies. In CACHE Challenge #1, 23 participants contributed their original workflows to identify small-molecule ligands for the WD40 repeat (WDR) of LRRK2, a promising Parkinson's target. We applied the FRASE-based hit-finding robot (FRASE-bot), a platform for interaction-based screening allowing a drastic reduction of the explorable chemical space and a concurrent detection of putative ligand-binding sites. In two screening rounds, 84 compounds were procured for experimental testing and 8 were confirmed to bind LRRK2-WDR with dissociation constants (Kd) ranging from 3 to 41 μM. To investigate the functional effect of WDR ligands, they were tested for their ability to modify the LRRK2 activity markers in HEK293T cells. Two compounds showed statistically significant increases in the kinase activity of WT LRRK2, and two compounds affected the conformation and kinase activity of major LRRK2 mutants.
Authors
- Hui Zeng (ORCID: https://orcid.org/0000-0002-7456-6061)
- P. Loppnau (ORCID: https://orcid.org/0009-0004-0002-355X)
- C. Arrowsmith
- Dmitry Kireev (ORCID: https://orcid.org/0000-0002-7896-2379)
- Levon Halabelian (ORCID: https://orcid.org/0000-0003-4361-3619)
- Albina Bolotokova
- Ian Chau (ORCID: https://orcid.org/0000-0003-0286-8703)
- Elisa Gibson (ORCID: https://orcid.org/0000-0002-7112-337X)
- S. Houliston
- Akhila Mettu (ORCID: https://orcid.org/0000-0002-0481-8235)
- Matthieu Schapira (ORCID: https://orcid.org/0000-0002-1047-3309)
- Li F
- Pegah Ghiabi
- Suzanne Ackloo (ORCID: https://orcid.org/0000-0002-9696-1839)
- Xiaolei Wang (ORCID: https://orcid.org/0000-0002-9750-1906)
- Shahnaz Perveen (ORCID: https://orcid.org/0000-0002-8667-8327)
- Ashley Hutchinson (ORCID: https://orcid.org/0000-0002-3481-8025)
- Marta Glavatskikh
- Harsh Vardhan J.
- Alma Seitova
- Lara Ordóñez A.J
Institutions
- University of North Carolina at Chapel Hill (US)
Publication Details
- Journal
- UNC Libraries
- Published
- 2025-02-12
- DOI
- https://doi.org/10.17615/qcft-3792
- Primary Topic
- Cytokine Signaling Pathways and Interactions
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Bristol-Myers Squibb
- Bayer
- University of Missouri
- Genome Canada
- Merck KGaA
- Ontario Genomics
- Ontario Genomics Institute
- Agence Nationale de la Recherche
- National Institutes of Health
- Genentech
- National Institute of General Medical Sciences