Exogenous bacterial cellulose induces plant tissue regeneration through the regulation of cytokinin and defense networks

Regeneration is a unique feature of postembryonic development extensively observed in plants. The capacity to induce regeneration exogenously is limited and usually confined to meristematic-like tissues. We show that bacterial cellulose (BC), but not other structurally similar matrixes, induces postwounding regeneration in nonmeristematic plant tissues via a distinctive route to callus-mediated regenerative programs. The BC-specific program involves cytokinin operating concurrently with strongly activated plant biotic response genes to induce plant regeneration. A reactive oxygen species (ROS) burst, normally associated with defense responses, is sustained upon BC application, involving a network of tightly interconnected transcription factors, where WRKY8, known for regulating stress responses, shows a clustering and hierarchical prevalence. WRKY8 regulates BC-mediated plant regeneration and ROS homeostasis, including superoxide anion accumulation, to potentially promote cell proliferation after wounding. Collectively, our results demonstrate that the cytokinin- and ROS-associated defense responses can be targeted by BC application to promote plant wound regeneration through alternative regenerative programs.