High‐Throughput Single‐Cell Analysis of Local Nascent Protein Deposition in 3D Microenvironments via Extracellular Protein Identification Cytometry (EPIC) (Adv. Mater. 6/2025)
Extracellular Protein Identification Cytometry Knowledge of the extracellular matrix drives our understanding of cell behavior. However, current analysis methods are limited to either bulk or low-throughput single-cell analysis, thus masking the heterogeneity in matrix deposition. Extracellular protein identification cytometry (EPIC) combines the high-throughput single-cell analysis of flow cytometry with engineered microniche 3D cell culture, jointly enabling in situ matrix analysis of large cell populations. More details can be found in article number 2415981 by Jeroen Leijten and co-workers.
Authors
- Alexis Wolfel (ORCID: https://orcid.org/0000-0002-4577-1699)
- Francisco Conceição (ORCID: https://orcid.org/0000-0003-4587-2470)
- Marieke Meteling (ORCID: https://orcid.org/0009-0006-3562-7921)
- Castro Johnbosco (ORCID: https://orcid.org/0000-0003-3617-4603)
- Kannan Govindaraj (ORCID: https://orcid.org/0000-0001-9030-8735)
- Jeroen Leijten (ORCID: https://orcid.org/0000-0002-8063-207X)
- Liliana Moreira Teixeira (ORCID: https://orcid.org/0000-0001-5039-8369)
Institutions
- University of Twente (NL)
Publication Details
- Journal
- Advanced Materials
- Published
- 2025-02-01
- DOI
- https://doi.org/10.1002/adma.202570044
- Primary Topic
- Advanced Biosensing Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00