GC-MS analysis of culture filtrates of Trichoderma harzianum and their efficacy of biocontrol measures of plant pathogens

Trichoderma species is a filamentous soil borne fungus known to be an effective bio control agent against plant pathogens.The chemical compositions of culture filtrates of Trichoderma harzianum were investigated using Perkin-Elmer Gas Chromatography-Mass Spectrometry, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library.Gas Chromatography -Mass Spectrometry analysis from T. harzianum showed five retention times 1. 91, 4.28, 4.52, 26.42 and 30.21 min.There, action time 1.91 corresponds to 3,6,9,12,15,18hexaoxa-1-triacontanolacid; 4.28corresponds to N-(tau-benzyl-N(1)-(tert-butoxycarbonyl) histidyl) serine methyl ester acid; 4.52 corresponds to didecyl phthalate acid; 4.22 corresponds to methoxy glutamic acid methyl ester; 4.32 corresponds to triacontanoic acid.