Amniotic Allograft with Coronally Advanced Flap for Treatment of Gingival Recession: A Randomized, Controlled Clinical Trial

<b>Objectives:</b> Amniotic membrane (AM) is an allograft obtained from humans that contains a variety of growth factors, and has outstanding healing qualities. This study aimed to assess the efficacy of amniotic allograft with coronally advanced flap (CAF) for root coverage. <b>Materials and Methods:</b> This randomized controlled clinical trial comprised 24 individuals with Miller's Class I and II root resorption defects. Each patient received treatment using the CAF approach, with the test group receiving a combined therapy using an AM. Measurements were made at baseline and 6 months after surgery for gingival biotype (GB), dentin hypersensitivity (DH), recession depth (RD), recession width (RW), gingival recession total surface area (GRTSA), keratinized tissue width (KTW) and probing depth (PD). <b>Results:</b> There was a statistically significant reduction in RD (from 2.83 mm to 0.92 mm), a significant increase in KTW (from 3.17 mm to 4.25 mm), improvement in GB, and a reduction in DH in the test group compared to the control group (P<0.05). <b>Conclusion:</b> Miller's Class I and II root recession defects can be effectively treated with a CAF. Application of AM under the CAF improved root coverage and GB, and further increased the KTW after 6 months, compared to CAF alone.