Precision Fabrication and Optimization of Nanostructures for Exosome Detection via Surface-Enhanced Raman Spectroscopy

Exosome detection is crucial for biomedical research and clinical diagnostics due to their unique characteristics. Surface-enhanced Raman spectroscopy (SERS) based on nanostructure substrates with local field enhancement capability is a promising detection approach. However, the random distribution of nanostructures leads to uneven "hotspots" distribution, which limits their application in SERS detection. Here, we systematically investigated the impact of experimental parameters on nanostructure morphology and analyzed their formation mechanism, achieving controllable nanocone fabrication. Subsequent experiments confirmed the reliability and effectiveness of the fabricated nanocone in exosome SERS detection. This work not only realized flexible control of nanostructures but also expanded their application prospects in the field of exosome analysis.