Nanochitosan Conjugated with Paromomycin: Preparation, Characterization and Medical Application

This study aims to prepare nanochitosan conjugated with paromomycin antibiotic and demonstrate its effect as a therapeutic agent against Entamoeba histolytica parasite in experimentally infected mice by studying the CD4+ and CD8+ lymphocyte cells. The nanomaterial was characterized using XRD examination, which indicated the formation of nanochitosan semicrystalline structure, including crystalline and amorphous. The biological results showed that CD4+ CD8+ had positive reactions. CD4+ was the most reactive biomarker than CD8+ that expresses the activation of T lymphocytes as a response to the E. histolytica infection, and these cells reduced as a response to the treatment showing the active effect of the Nanomaterial as a therapeutic agent against Entamoeba histolytica, parasite, this study is the first in its field to determine the effect of nanochitosan on lymphocytes in Entamoeba histolytica parasitic infection.