Broadly Targeted Metabolomics Analysis of Differential Metabolites Between Bupleurum chinense DC. and Bupleurum scorzonerifolium Willd.

<b>Background/Objectives:</b> Bupleuri Radix is a plant in the Apiaceae family <i>Bupleurum Chinense</i> DC. or <i>Bupleurum scorzonerifolium</i> Willd. root. The dissimilarities in the metabolite profiles of plants directly correlate with the disparities in their clinical efficacy. <b>Methods</b>: Therefore, the wild <i>Bupleurum Chinense</i> DC. (YBC) and wild <i>Bupleurum scorzonerifolium</i> Willd. (YNC) were used as research materials. They were analyzed using the UPLC-MS/MS and the similarities and differences were uncovered based on differential metabolites. <b>Results:</b> Our results proved that the differences in clinical efficacy between YBC and YNC may be attributed to their distinct metabolite profiles, as follows: (1) a total of 12 classes of 2059 metabolites were identified in the roots, with phenolic acids, terpenoids, and flavonoids being the most abundant metabolic products, with 2026 shared components between the two, 2045 in YBC, and 2040 in YNC; (2) a total of 718 differential metabolites were identified, accounting for 35.44% of the shared metabolites. Among them, YBC had 452 metabolites with higher content relative to YNC, representing 62.95%, and 266 components with lower content, representing 37.05%; (3) the KEEG enrichment analysis results show that the differential metabolic pathways are flavone and flavonol biosynthesis, linoleic acid metabolism, arachidonic acid metabolism, sesquiterpenoid and triterpenoid biosynthesis, and linolenic acid metabolism. <b>Conclusions:</b> These new findings will serve as a foundation for further study of the BR biosynthetic pathway and offer insights into the practical use of traditional Chinese medicine in clinical settings.