IN VITRO RESPONSE OF COTINUS COGGYGRIA SCOP. TO THE SPETRAL RADIATION AND LEDS INTENSITY

Background. Modern wide-spectrum LED installations' application under strictly controlled conditions with the correct choice of the quantitative and qualitative light composition helps the microclonal propagation of species Cotinus coggygria Scop., used in agroforestry, landscaping, and pharmaceuticals. Purpose. Choosing the best photomorphogenesis parameters in vitro on a hormone-free medium for C. coggygria as a perspective plant species for commercial uses. Materials and methods. The effect of 15 lighting options, namely 6 ratios of red (R), blue (B), and green (G) spectra, on the composition of LEDs on C. coggygria explants in vitro by methods used in microclonal propagation was studied. The biochemical parameters’ content in the samples of the studied leaves was determined using the optical method. Statistical data processing was carried out using the Statistica 12.0 application package (StatSoft, USA). Results. Determining the parameters of regenerants and leaf plates cultivated in vitro, as well as the pigment leaves’ composition, made it possible to select the optimal spectral ratio (1R:1B:0.5G) and PPFD values (40–70 µmol m-2 s-1) to obtain maximum results at cultivation on a hormone-free medium according to the Murashige and Scoog protocol. During the analysis of C. coggygria biochemical leaf composition, the maximum total chlorophyll amounts (17-21.5 μg cm2) and NBI (67.8-71.0 с.u.) and the minimum flavonoids and anthocyanins amounts were obtained, which confirms the normal photosynthetic’ apparatus work of the studied microshoots. Conclusion. The results obtained can be recommended to be used for optimizing the micropropagation technology of C. coggygria. Funding. The research was carried out within the framework of the state assignment of the Ministry of Science and Higher Education of the Russian Federation No.122020100427-1 To develop a scientific basis for the preservation and reproduction of valuable wood and shrubs genotypes in culture in vitro.