<scp>UHPLC</scp>‐<scp>HRMS</scp>/<scp>MS</scp>–Based Metabolic Profiling and Quantification of Phytochemicals in Different Parts of <scp><i>Coccinia grandis</i></scp> (L.) Voigt

<i>Coccinia grandis</i> (L.) Voigt (<i>C. grandis</i>), a member of the Cucurbitaceae family, is recognized for its phytochemicals that possess antioxidant and antidiabetic properties, along with a wide array of nutritional and health-promoting benefits. However, a comprehensive investigation of the phytochemical profiles and biologically active constituents in different parts of <i>C. grandis</i> has not yet been reported. Therefore, this study aimed to evaluate the phytochemical constituents of three distinct parts of <i>C. grandis</i> (fruit, leaves, and stem) at the same growth stage. The phytochemicals in <i>C. grandis</i> were identified using UHPLC-HRMS-based untargeted metabolomics, followed by a quantitative analysis of the primary metabolites. The qualitative analysis revealed 60 secondary metabolites, including phenolic compounds (6 hydroxybenzoic acids, 22 hydroxycinnamic acids, 2 coumarins, 1 flavanone, 1 flavanonol, 2 flavones, 22 flavonols, and 2 lignans) and triterpenes (2 cucurbitacins). Furthermore, nine plant hormones and 30 amino acids were successfully identified. The quantitative analysis of 32 types of secondary metabolites indicated that the leaves contained the highest total amounts of flavonoids (501.37 mg/100 g) and hydroxycinnamic acids (1148.23 mg/100 g). Additionally, the analysis of amino acids revealed a total of 20 types, with the leaf extract exhibiting the highest total amounts of both essential and nonessential amino acids, followed by the fruit and stem extracts. In conclusion, the analysis of the primary and secondary metabolite composition and content of various parts of <i>C. grandis</i> demonstrated that the leaf extract replace with had the greatest functionality, suggesting its potential utility in the development of health functional foods.