Allelic Variation in HMW-GS of Mongolian Wheat Varieties Using KASP Molecular Marker

The storage proteins in wheat, particularly the high molecular weight glutenin subunits (HMW-GS), play crucial roles in the processing of flour and the quality of bread made from common wheat.These subunits are encoded by the Glu-A1, Glu-B1, and Glu-D1 loci.This study aimed to determine the composition of high molecular weight glutenin subunits in 89 Mongolian cultivars of spring wheat using KASP markers and sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE).The result showed the presence of the 5+10 allele at the Glu-D1 locus in Mongolian-developed varieties was confirmed through SDS-PAGE, providing evidence for the synthesis of high molecular weight glutenin subunits.The uniform presence of high molecular weight glutenin subunit alleles across the tested cultivars contributes valuable insights into the bread-making quality of Mongolian wheat varieties.