Nanopore adaptive sampling to identify the NLR gene family in melon (Cucumis melo L.)
Overall, we demonstrated that NAS is a simple and efficient approach for exploring complex genomic regions, such as clusters of Nucleotide-binding site leucine-rich repeat (NLR) resistance genes. These regions are characterized by containing a high number of copy number variations, presence-absence polymorphisms and repetitive elements. These features make accurate assembly challenging but are crucial to study due to their central role in plant immunity and disease resistance. This approach facilitates resistance gene characterization in a large number of individuals, as required when breeding new cultivars suitable for the agroecological transition.
Authors
- Stéfan Engelen (ORCID: https://orcid.org/0000-0003-0003-1192)
- Vincent Rittener-Ruff
- William Marande (ORCID: https://orcid.org/0009-0009-1277-1754)
- Rafael Feriche-Linares
- Damien Daniel Hinsinger (ORCID: https://orcid.org/0000-0001-7459-7610)
- Nathalie Boissot (ORCID: https://orcid.org/0000-0002-8266-9386)
- Corinne Cruaud (ORCID: https://orcid.org/0000-0002-4752-7278)
- Patricia Faivre Rampant (ORCID: https://orcid.org/0000-0003-0777-6621)
- Isabelle Le-Clainche
- Aurélie Canaguier (ORCID: https://orcid.org/0000-0001-8674-4622)
- Javier Belinchon-Moreno (ORCID: https://orcid.org/0009-0006-6110-6767)
- Jacques Lagnel
- Aurélie Berard
- Véronique Chovelon
Institutions
- Centre National de la Recherche Scientifique (FR)
- Commissariat à l'Énergie Atomique et aux Énergies Alternatives (FR)
- Université Paris-Saclay (FR)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Genoscope (FR)
- CEA Paris-Saclay (FR)
- Génétique et amélioration des fruits et légumes (FR)
- Laboratoire de Recherche en Sciences Végétales (FR)
Publication Details
- Journal
- BMC Genomics
- Published
- 2025-02-10
- DOI
- https://doi.org/10.1186/s12864-025-11295-5
- Citations
- 5
- Primary Topic
- Plant Pathogenic Bacteria Studies
- Type
- article
- Field-Weighted Citation Impact
- 8.75
Funders
- Avignon Université
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement