COMPARATIVE INSIGHTS INTO ULTRAVIOLET-B RADIATION-INDUCED BIOCHEMICAL MODULATIONS IN SENNA AURICULATA: A GAS CHROMATOGRAPHY-MASS SPECTROMETRY PROFILING STUDY

Objectives: This study explores the transformative effects of ultraviolet B (UV-B) radiation on the phytochemical profile of Senna auriculata leaves, a plant known for its medicinally properties. Methods: Utilizing gas chromatography-mass spectrometry, we systematically compared the phytochemical profiles of methanolic extracts from untreated leaves (SKC) and those exposed to UV-B radiation (SKT). Results: The analysis revealed a total of 59 compounds in the untreated leaves (SKC) and 50 in the UV-B exposed leaves (SKT). Among these, 36 metabolites were common to both samples, while 23 were unique to SKC, and 14 were exclusive to SKT. Notable compounds induced by UV-B radiation included Cystamine, 2-Methyl-6-(5-methyl-2-thiazolin-2-ylamino) pyridine, and 1,2-Benzisothiazol-3-amine tbdms. These compounds exhibited significant bioactivities, demonstrating antioxidant, anticancer, and antibacterial properties. Conclusion: The findings highlight the role of UV-B radiation as a modulator of secondary metabolism, reshaping the phytochemical profile of S. auriculata to enhance its adaptive resilience and therapeutic potential. This study sheds light on the complex interaction between environmental stressors and phytochemistry, providing valuable insights into how controlled UV-B exposure can optimize the medicinal properties of plants.