The effect of thyme essential oil on duodenal toxicity induced by subacute exposure to voliam targo® insecticide in male rabbits

The increasing use of pesticides has raised concerns about their gastrointestinal toxicity, leading to the search for natural remedies such as thyme essential oil. For that, this study aimed to determine the protective effect of Thymus vulgaris essential oil (TEO) with its chemical composition against Voliam Targo-induced duodenal toxicity. Twenty male rabbits were randomly assigned to four equal groups and treated for 21 consecutive days: Control, VT insecticide group, TEO essential oil group, and VT + TEO group. The main constituent of the essential oil of T. vulgaris was carvacrol 72.9 %. The duodenal injury was assessed using biochemical, histomorphometrical, and immunohistochemical methods. The VT induced an increased number of benign intestinal tissue changes, such as hyperplasia of Brunner glands, disorganization of villi, and infiltration of inflammatory cells. The co-administration of TEO with VT restored the histological organization of the duodenum. In addition, the immunohistochemical examination of the duodenal tissues shows positive immunostaining for the expression of Ki67, P53, and BCL2 proteins in the VT group. Lower expressions were noted in the VT-TEO group compared to the control and TEO groups. The E-cadherin and β-catenin immuno-signals were significantly higher in the essential oil treatment groups' duodenal sections than in the VT group. The study suggested that VT caused duodenal toxicity and that the carvacrol chemotype of TEO could mitigate and alleviate this effect.