Comparative analysis of human telomerase reverse transcriptase mutation in transitional epithelial cell bladder cancer in high and low grade tumors

Aims: We aimed to evaluate the presence of TERT promoter region mutations in transitional epithelial cell bladder cancer by analyzing the h-TERT mutation in high and low-grade tumors and to compare it with the clinicopathological data of the patients. Methods: A total of 90 patients diagnosed with bladder cancer, 60 with low-grade tumors and 30 with high-grade tumors were included in this study. To detect mutations in the TERT gene in the DNA samples obtained, the most frequently mutated region of the gene was amplified by PCR using forward and reverse primers and mutation analysis was performed by sequence analysis. Results: TERT promoter mutation was positive in 38 and negative in 22 out of 60 patients in the low grade group, and positive in 26 and negative in 4 out of 30 patients in the high grade group. Patients with high-grade tumors were 1.940 times more likely to be TERT promoter mutation positive than low-grade patients (p=0.027) Conclusion: When interpreted together with other studies that have obtained significant results that TERT mutation analysis can be used in diagnosis, tumor grading and follow-up, it is thought that it can be used in the grading and follow-up algorithm.