The Effect of Jeopgol-Tang 2.0 on Recovery of Bone Fracture in Sprague-Dawley Rats

Natural Korean medicine has already been a great success, being used to treat various types of othopedic diseases, especially fracture and osteoporosis. In this study, we find a new mixture of herbal medicine (JGT 2.0) of five well-known Korean herbal medicine for bone healing, including Angelica gigas, Dendrobium moniliforme, Dipsaci Radix, Antler, Astragalus membranaceus, Conidium officinale, Achyranthes japonica, Messa medicate fermentata on bone fracture healing. To evaluate the efficacy of JGT 2.0, the ulna of Sprague-Dawley rats was intentionally fractured and administered JGT 2.0, followed by radiographic imaging and histological examination using H&E staining to confirm the progress of recovery over time at the fracture site. X-ray imaging showed no significant difference between groups up to 1 week after JGT 2.0 administration. However, after 4 weeks of JGT 2.0 administration, bone regeneration decreased by -15% in the control group, but increased by 23.6% in the JGT 2.0 group. In addition, the length of calluses increased by 252% in the JGT 2.0 group compared to the control group. Taken together, the results suggest that JGT may offer a novel therapeutic approach to accelerate the growth of bone calluses and promote healing of bone fractures.