Novel Method for the Estimation of Minoxidil and Aminexil in Marketed Formulation

The goal is to provide a simple, accurate, and sensitive HPTLC technique for estimating Minoxidil and Aminexil in topical solutions. Silica gel 60 F254 pre-coated TLC aluminium plates were used as the stationary phase in the procedure. Methanol: water (5:5) was the solvent system in use. At 254 nm, densitometric analysis was performed. The results showed that the Rf values for Aminexil and Minoxidil were 0.88 and 0.69, respectively. According to the findings, the scanning wavelength was 254 nm and was linear between 2 and 10 μg/spot for minoxidil and 6–14 μg/spot for aminexil. The %RSD was less than 2%. Minoxidil and Aminexil I were shown to have regression coefficients of 0.9983 and 0.9993, respectively. A simple, accurate, precise, and sensitive HPTLC approach is designed and validated in compliance with ICH criteria for measuring minoxidil and aminexil in combination in topical solution.