Dafadine Does Not Promote Dauer Development in Pristionchus pacificus

In response to unfavorable conditions, nematodes develop into the stress-resistant dauer larvae. Under favorable conditions, many nematodes are known to synthesize dafachronic acids (DAs) that bind to the conserved nuclear hormone receptor DAF-12 to suppress dauer development. However, the enzymes involved in the production of DAs have not been thoroughly investigated in <i>Pristionchus pacificus</i> . Here we show that the cytochrome P450 inhibitor Dafadine-A, which suppresses DAF-9 in DA biosynthesis in <i>C. elegans</i> and other nematode species, does not cause constitutive dauer formation or gonad migration defects in <i>P. pacificus</i> wild type. Instead, Dafadine-A may slightly reduce <i>P. pacificus</i> growth rate.