Green Analytical Chemistry Based Quality by Design Assisted UV Spectroscopic Method Development and Validation for Estimation of Chrysin

The objective of current research work was to integrate Quality by Design (QbD) approach with the principles of green analytical chemistry with a focus on minimizing detrimental environmental effects. A cost-efficient UV spectroscopic method was developed and validated as per ICH guidelines for chrysin analysis. Water was used as a major solvent. Sonication time and scanning interval were chosen as independent variables and absorbance was selected as dependent variables. DoE was used to optimize trial runs. To identify the optimized spectroscopic method, a simple 3² factorial design with 2 factors and 2 levels was employed, resulting in 9 experimental runs. The optimized run was extensively validated as per ICH guidelines. A linear relationship was determined between the concentrations ranging from 2 to 10 µg/ml, with a regression coefficient of 0.998. Both precision assays showed a % RSD less than 2 indicating the precise nature of the method. The percentage recovery ranged from 99.84 to 101.26% indicating promising accuracy. Sensitivity of the proposed method is proved by its LOD and LOQ values which are 0.51 μg/mL and 1.57 μg/mL respectively. The % RSD less than 2 indicates that the developed method is rugged and robust. Additionally, the method exhibited promising repeatability. Hence, it can be concluded that integration of QbD and Green chemistry offers a promising avenue for developing a cost-effective and environment friendly analytical method for chrysin analysis.