Protocol to study chloride regulation in cultured mouse cortical neurons using electrophysiology
Inhibitory synaptic transmission mediated by the neurotransmitter γ-aminobutyric acid (GABA) is dependent on the concentration of chloride ions (Cl<sup>-</sup>) in neurons, which can be assessed by making patch-clamp recordings of the reversal potential for GABA (E<sub>GABA</sub>). Here, we present a protocol to study the regulation of cation-chloride cotransporters and the strength of synaptic inhibition in cultured mouse cortical neurons using electrophysiology. We describe steps for culturing neurons isolated from postnatal pups and electrophysiological measurement of E<sub>GABA</sub>. For complete details on the use and execution of this protocol, please refer to Raveendran et al.<sup>1</sup>.
Authors
- Jessica C. Pressey (ORCID: https://orcid.org/0000-0002-3898-6579)
- Melanie A. Woodin (ORCID: https://orcid.org/0000-0003-2984-8630)
- Vineeth A. Raveendran
Institutions
- University of Toronto (CA)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-02-08
- DOI
- https://doi.org/10.1016/j.xpro.2025.103628
- Primary Topic
- Neuroscience and Neuropharmacology Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- University of Toronto
- Canadian Institutes of Health Research
- Natural Sciences and Engineering Research Council of Canada